백서의 하악골 결손부 치유시 골형성단백질 2/4의 발현에 관한 연구
Immunohistochemical Study for the Expression of BMP-2/4 on the Healing Process in the Mandibular Bone Defect or Rat
최재용, 이상철,
소속 상세정보
최재용 ( ) - 경북대학교 치과대학 구강내과학교실
이상철 ( ) - 경북대학교 치과대학 구강내과학교실
KMID : 0355620000260040325
Abstract
-Abstract-
Bone morphogenetic protein-2/4 are members of Transforming Growth Factor-β
(TGF-β) superfamily and they may induce formation of cartilage and bone in vivo.
This study was performed to investigate the cellular target and period of action of
BMP-2/4 and understanding of actions of BMP-2/4 at cellular level. The appearance of
BMP-2/4 during healing of mandibular and periodontal defect in rat was evaluated
immunohistochemically. 40 Sprague-Dawley strain white male rats, each weighing about
300gm were used. Bony defect was performed in the mandible and they were sacrificed
at the day of 3rd, 10th, 20th, 30th after operation. The specimens were harvested and
examined histologically and immunohistochemically by localization of anti-BMP-2/4.
The results were as follows :
1. Woven bone was observed at 10 day and perfect healing of defect with compact
bone and periodontal ligment space at 30th day.
2. Osteoprogenitor cells, osteoblastic cells and periosteum were positive reaction to
immunohistochemical stain at 10th day.
3. Cells of bone marrow space and surface cells of osteocytes and cementoblasts were
positive reaction to immunohistochemical stain at 20th day.
4. Newly formed osteocytes and cementosytes were positive reaction to
immunohistochemical stain at 30th day.
From the above findings, we could conclude that BMP-2/4 acted significant roles as
factors of induction, proliferation and differentiation during bone healing process.
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